The efficiency depends on the isotope and instrumentation. If you are using a molecule labeled with 125I, the specific activity equals 2200 Ci/mmol if each molecule is labeled with one iodine.Īlso enter the counter efficiency - the fraction of radioactive disintegrations that are detected. The result is the volume of stock you need to use.Įnter the specific radioactivity as Ci/mmol which should be on the label. Also enter the concentration and volume you want. The calculations assume that the decay product is not biologically active, so the concentration of stock that is biologically active decreases over time.Įnter the concentration in your stock solution, after accounting for decay. If you are using a molecule labeled with 125I, the specific activity equals 2200 Ci/mmole if each molecule is labeled with one iodine.Īlso enter the percent of the original isotope remaining (calculated above). Select one of the common isotopes, or enter the half-life of another isotope.Įnter mCi/ml and Ci/mmole, which should be on the label. Calculates radioactive decay during a specified number of days.
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